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Literature summary extracted from

  • Bardy, S.L.; Jarrell, K.F.
    Cleavage of preflagellins by an aspartic acid signal peptidase is essential for flagellation in the archaeon Methanococcus voltae (2003), Mol. Microbiol., 50, 1339-1347.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.23.52 expressed in BL21(DE3)/pLysS membranes Methanococcus voltae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.23.52 D186A the mutation does not affect the activity towards preflagellin FlaB2 Methanococcus voltae
3.4.23.52 D18A inactive Methanococcus voltae
3.4.23.52 D18E the mutation results in an apparently unstable protein Methanococcus voltae
3.4.23.52 D18N inactive Methanococcus voltae
3.4.23.52 D190A the mutation does not affect the activity towards preflagellin FlaB2 Methanococcus voltae
3.4.23.52 D224A the mutation does not affect the activity towards preflagellin FlaB2 Methanococcus voltae
3.4.23.52 D79A inactive Methanococcus voltae
3.4.23.52 D79E the mutant restores preflagellin peptidase activity (despite a reduced amount of peptidase produced) Methanococcus voltae
3.4.23.52 D79N inactive Methanococcus voltae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.23.52 membrane
-
 Methanococcus voltae 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.52 25900
-
 x * 25900, calculated from amino acid sequence Methanococcus voltae

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.52 Methanococcus voltae
-
-
-
3.4.23.52 Methanococcus voltae PS
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.52 preflagellin FlaB2 + H2O
-
 Methanococcus voltae flagellin FlaB2 + ?
-
 ?
3.4.23.52 preflagellin FlaB2 + H2O
-
 Methanococcus voltae PS flagellin FlaB2 + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.52 ? x * 25900, calculated from amino acid sequence Methanococcus voltae

Synonyms

EC Number Synonyms Comment Organism
3.4.23.52 FlaK
-
 Methanococcus voltae
3.4.23.52 RMVO00262 recombinant enzyme Methanococcus voltae

General Information

EC Number General Information Comment Organism
3.4.23.52 physiological function the enzyme plays an essential role in flagellation Methanococcus voltae